RAPD Analysis to Genetic Diversity Between Inbred Lines and Its′ Hybrid Progenies F_1 in Cucumber(Cucumis sativus L.)

This study detected genetic differences between cucumber(Cucumis sativus L.) inbred lines and its′ hybrid progenies F_1 from molecular level by means of RAPD marker combing field experiment.A total of 30 primers were employed for PCR amplification of 11 genotype of cucumbers,from which 167 polymorphic loci were stored,about 71.37% of total loci.The polymorphic products amplified by each primer ranged from 2-7,with an average of 7.25.According to RAPD genetic distance matrix,cluster analysis was carried out by UPGMA method.The result showed that the hybrid progenies F_1 were inclined to female inbred lines in heredity.Genetic distance among parents inbred lines ranges from 0.123 to 0.164.The result showed that the genetic differences of cucumber were small.As male,F_1 inherits more genetic characteristics of Jinyan 7 inbred lines than Ningyangdaci inbred lines.The above results are roughly concordant with field experimental result and actual situation.It is feasible for RAPD to use for studying genetic diversities of cucumber.The research result can act as theoretical basis for parental selection and molecular marker-assisted selection in breeding procedures.