2012•Chinese Journal of Health Laboratory TechnologyRequires access

PFGE technology for study on homology of Vibrio parahaemolyticus strains in a food poisoning event

Rui Zhang

Abstract

Objective:To understand the homology of the Vibrio parahaemolyticus(VP) strains isolated from a food poisoning.Methods:VP was isolated and identified according to the national standard.The isolated VP strains were carried out serotyping and virulence gene(tdh,trh) detection,then pulsed-field gel electrophoresis(PFGE) was used for genotyping.Results:11 VP strains with the serotype of O3:K6 were isolated from 10 patients and a chopping block.In this detection,all the VP isolates carried tdh virulence genes and had the same PFGE pattem.Conclusion:This is a food poisoning caused by VP pollution in the food processing link.

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Key concepts: Pulsed-field gel electrophoresis, Vibrio parahaemolyticus, Serotype, Virulence, Genotyping, Microbiology, Food poisoning, Biology