Study on Extraction and Purification Technology for Lentinan

Complex enzyme method was applied to extract lentinan from shiitake mushroom(Lentinus edodes),based on single-factor experiment,factors as decolorization temperature,amount of active carbon,decolorization time,and pH value were tested in orthogonal design,optimum decolorazation technology was selected,additionally,the decolorized lentinan was purified by combination of enzymatic and sevage combination for deproteinization.The results showed that the optimum condition was decolorization temperature 20℃,amount of active carbon 1.0%,decolorization time 90min,and pH 3.Under these conditions,decolorization rate of lentinan could reach 71.3% with the lowest polysaccharide loss rate of 7.8%,and the color of decolorized and purified lentinan was white with protein removal rate of90.26%.