Extraction of Polysaccharide in Lentinus edodes by Enzymatic Method
Qianqian Liu
Abstract
Qianqian Liu
Abstract
Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.
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Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.
Key concepts: Papain, Cellulase, Lentinan, Lentinus, Chemistry, Extraction (chemistry), Enzymatic hydrolysis, Chromatography