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Extraction of Polysaccharide in Lentinus edodes by Enzymatic Method

Qianqian Liu

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Abstract

Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.

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What this paper is about

Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.

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Available abstract

Papain and cellulase were applied on the extraction of lentinan,and the extraction conditions of enzymatic method were studied in this research.The results showed that the optimal conditions of papain were enzymatic concentration 0.5%,temperature 50 ℃,pH6~7 and reaction time 1 h.The optimal conditions of cellulase were enzymatic concentration 0.25%,temperature 40 ℃,pH4.5~5.0 and reaction time 1 h.By enzymatic hydrolysis,the extraction yield of lentinan was improved distinctly.

Key concepts: Papain, Cellulase, Lentinan, Lentinus, Chemistry, Extraction (chemistry), Enzymatic hydrolysis, Chromatography

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