Study on Genetic Diversity of Male Ginkgo biloba L

[Objective] The research aimed to analyze the genetic diversity of germplasm resources of male Ginkgo biloba L so as to lay foundation for its conservation and breeding innovation. [Method] The improved CATB method was adopted to extracted genomic DNA in the blades of male ginkgo from 11 provinces, the random primers were used for RAPD amplification after primary and second screening, and genetic diversity of germplasm resources of male ginkgo was analyzed by POPGENE software. [Result] 12 pairs of premiers with clear amplified bands, good repeatability and polymorphism were screened out from more than 100 pairs of random primers for RAPD amplification and analysis of genetic diversity. 12 pairs of RAPD premiers that were screened out were used for PCR amplification on 40 samples, and 96 clear bands were obtained, 41 bands of which had polymorphism. The mean number of effective alleles of germplasm resources of male ginkgo was 1.644 0, the average gene diversity was 0.375 2 and average Shannon information index was 0.556 2. [Conclusion] Germplasm resources of ginkgo had abundant genetic diversity, which had important significance for its effective preservation and reasonable utilization.