Genetic diversity analysis of 32 Ginkgo varieties by RAPD

The leaves of 32 ginkgo cultivars(Ginkgo biloba L.) were used to extract genomic DNA.8 single primers and 4 primer pairs were selected from 56 random primers for RAPD amplification.RAPD amplification on agarose gel was used for studies on genetic diversity and NTSYS-pc 2.10e software was used to calculate the Dice similarity coefficient,and dendrogram was constructed using UPGMA method based on Dice similarity coefficient.The results showed that 85 amplified loci were detected,of which 81(95%) were polymorphic.It has been proved that the 32 samples presented much higher genetic diversity on DNA molecular level.They may be divided into 2 groups.The cultivars including Beijing-meihe,Nanxiong-shangdai,Huakou-dabaiguo were in one group,and the others were in another group.In the four Taixing cultivars,Taixing No.2 and Taixing No.3 have 0.968 average similarities,showing that they were low genetic diversity and implying that they may belong to the same cultivars.