The proteetive effects of ligustrazine on ischemia-reperfusion myocardial injury in isolated rat hearts

Objective To observe the protective effects of ligustrazine on ischemia-reperfusion myocardial injury in isolated rat hearts. Methods In the model of ischemia and reperfusion-induced myocardial injury: The isolated rat hearts were perfused in a Langendorff model. All hearts, except those of the control groups, were equilibrated for 20 min before treatment with 20 min of global ischemia and 40 min of reperfusion. A water-filled latex balloon connected to a pressure transducer was inserted into the left ventricle through the left atrium. The recorded by a computer. Left ventricular pressure(LVP), the first derivatived of left ventricular pressure(±dp/dtmax)and heart rate(HR),were continously monitored. Coronary flow(CF)was neasured by timed collection of coronary effluent. Myocardial injury was monitored by assaying creatine kinase(CK)release from the heart. The activity of CK in the coronary effluent at 5 min of reperfusion was measured spectrophotometrically. Results Ischemia-reperfusion can reduce the coronary flow and the baseline of LVP. Results Ischemia-reperfusion can reduce the coronary flow and the baseline of LVP,±dp/dtmax, after ischemia for 20 min, a decline in cardiac function and an increase in the release of CK were shown during reperfusion. Ligustrazine(40-80mg·L-1)markedly improved heart function, in crease the coronary flow and reduced the release of creatine kinase. Conclusion Ligustrazine possess a protective effect of myocardium against ischemia-reperfusion damage.