Simultaneous Shenfu injection treatment during reperfusion mitigates ischemia/reperfusion myocardium injury in isolated rat hearts

Objective To investigate the effect of simultaneous treatment with Shenfu injection(SF) during reperfusion on myocardial ischemia/reperfusion injury in rats.Methods The myocardial ischemia/reperfusion model was prepared based on the Langendorff model for the perfusion of isolated rat heart.Following ischemia,reperfusion was allowed with SF injection administered simultaneously.The hemodynamic variables,such as left ventricular end-diastolic pressure(LVEDP),left ventricular developed pressure(LVDP),rate of left ventricular pressure development/relaxation(±dp/dtmax) and heart rate(HR),and the content of adenosine triphosphate(ATP),malendialdehyde(MDA),superoxide dismutase(SOD) in myocardial tissue and the activities of creatine kinase(CK) in coronary effluent fluid were observed.The isolated perfused rat hearts were separated into control,ischemia/reperfusion,SF 30 ml/L and SF 60 ml/L groups.Results In the ischemia/reperfusion group,the content of ATP and activity of SOD significantly decreased;the activities of MDA and CK significantly increased.SF 60 ml/L attenuated the decrease of SOD and the increase of MDA and CK compared with the ischemia/reperfusion group.SF 60 ml/L significantly improved the recovery of the hemodynamic variables and myocardial ATP concentrations following ischemia and reperfusion.Conclusion Simultaneous SF treatment during reperfusion could provide the cardiac protection effect against the ischemia/reperfusion injury by increasing the content of ATP and activity of SOD and decreasing the activity of MDA and CK in myocardial.