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Content Determination of Ginsenoside Re in Panax ginseng by UPLC

Sun Quan-le

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Abstract

OBJECTIVE:To develop the method for the content determination of ginsenoside Re in Panax ginseng.METHODS:UPLC method was adopted.The determination was performed on Waters patened hybrid particle column(50 mm×2.1 mm,1.7 μm) with mobile phase consisted of acetonitreile-water(19:81) with detection wavelength at 203 nm.RESULTS:The linear range of ginsenoside Re was 0.531～1.239 μg·mL-1(r=0.999 8) with an average recovery of 98.70%(RSD=1.23%,n=6).CONCLUSION:The method is simple,convenient and accurate with high efficacy,and it can be used for the content determination and quality control of P.ginseng.

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What this paper is about

OBJECTIVE:To develop the method for the content determination of ginsenoside Re in Panax ginseng.METHODS:UPLC method was adopted.The determination was performed on Waters patened hybrid particle column(50 mm×2.1 mm,1.7 μm) with mobile phase consisted of acetonitreile-water(19:81) with detection wavelength at 203 nm.RESULTS:The linear range of ginsenoside Re was 0.531～1.239 μg·mL-1(r=0.999 8) with an average recovery of 98.70%(RSD=1.23%,n=6).CONCLUSION:The method is simple,convenient and accurate with high efficacy,and it can be used for the content determination and quality control of P.ginseng.

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Available abstract

OBJECTIVE:To develop the method for the content determination of ginsenoside Re in Panax ginseng.METHODS:UPLC method was adopted.The determination was performed on Waters patened hybrid particle column(50 mm×2.1 mm,1.7 μm) with mobile phase consisted of acetonitreile-water(19:81) with detection wavelength at 203 nm.RESULTS:The linear range of ginsenoside Re was 0.531～1.239 μg·mL-1(r=0.999 8) with an average recovery of 98.70%(RSD=1.23%,n=6).CONCLUSION:The method is simple,convenient and accurate with high efficacy,and it can be used for the content determination and quality control of P.ginseng.

Key concepts: Ginseng, Chromatography, Ginsenoside, Content determination, Chemistry, High-performance liquid chromatography, Linear range, Linear relationship

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