Study on Male-Sterility in Zea mays VIII. RFLP Analysis of mtDNA of Y_(II-1) Type Maize Male-Sterile Cytoplasm

One set of male sterile lines with the same nuclear backgrounds in maize Cms T U8112、 Cms s U8112、 Cms C U8112 and Cms Y Ⅱ 1 U8112 were used in our experiment, mtDNA was prepared according to Kemble, R. J.; Hybridization had been described by Sambrook, J., Long length agrose gel electropherosis can bring out high resolution for Eco RⅠ, Hind Ⅲ, Bam HⅠ and Past Ⅰ restricted fragments of mtDNA of corn T、 S、 C group and new Y Ⅱ 1 type male sterile cytoplasms. Hybridization of 5 mtDNA specific probes to restricted mt DNA shown that many different hybridized bands were existed among T、 S and C groups, Y Ⅱ 1 type was difference from T and S group, but there was tiny difference with C group in hybridized bands. We would apply the technique of sequence analysis to the division of masle sterile Y Ⅱ 1 type.