Zhao‐Hui Wu
Abstract
To study enzymatic hydrolysis conditions in honeybee, papain was used to study the effects of temperature, time, enzyme amount, pH value and solid-liquid ratio on hydrolysis with orthogonal experiment. The results showed that the optimal conditions is temperature 50 ℃,3 h,pH 6.5,enzyme amount 2.4%, solid-liquid ratio 1∶3.
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To study enzymatic hydrolysis conditions in honeybee, papain was used to study the effects of temperature, time, enzyme amount, pH value and solid-liquid ratio on hydrolysis with orthogonal experiment. The results showed that the optimal conditions is temperature 50 ℃,3 h,pH 6.5,enzyme amount 2.4%, solid-liquid ratio 1∶3.
Key concepts: Papain, Hydrolysis, Enzymatic hydrolysis, Enzyme, Chemistry, Chromatography, Organic chemistry