Effect of different growth regulators on callus induction from explants in Creeping Dichondra
Tian Zhi-hong, Xiaoli Li, Yan Han, Qiu YongFu, Yong He
Abstract
Tian Zhi-hong, Xiaoli Li, Yan Han, Qiu YongFu, Yong He
Abstract
Creeping Dichondra (Dichondra repens) may be considered as a kind of excellent turfgrass and ground cover plants. This experiment inspected the effect of four different growth regulators on callus induction of cotyledon, young leaf, petiole and hypocotyl in Creeping Dichondra with the orthogonal design. The results showed that growth regulator was an important factor on induction of callus, and 2,4-D was essential to induction of callus. The optimum medium supplemented with growth regulators for callus induction of Creeping Dichondra was MS+1.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.2 mg/L KT+1.0 mg/L α-NAA.
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Creeping Dichondra (Dichondra repens) may be considered as a kind of excellent turfgrass and ground cover plants. This experiment inspected the effect of four different growth regulators on callus induction of cotyledon, young leaf, petiole and hypocotyl in Creeping Dichondra with the orthogonal design. The results showed that growth regulator was an important factor on induction of callus, and 2,4-D was essential to induction of callus. The optimum medium supplemented with growth regulators for callus induction of Creeping Dichondra was MS+1.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.2 mg/L KT+1.0 mg/L α-NAA.
Key concepts: Callus, Petiole (insect anatomy), Cotyledon, Explant culture, Hypocotyl, Botany, Growth regulator, Plant growth