Effects of non-wounded ischemic preconditioning on myocardium ischemic reperfusion injury in rats

AIM:To determine whether the non-wounded legs repeated-brief ischemic preconditioning has protective effects to extend the further application of IPC.METHODS:Two animal models which were the non-wounded legs repeated-brief ischemic preconditioning and the classical ischemic preconditioning,were produced to be compared with their protective effects on myocardial ischemic-reperfusion injury.Rats were divided into four groups:normal control group (NC, n =8),ischemic-reperfusion group (I/R, n =12), calssical ischemic preconditioning(C-IPC, n =12), and non-wounded legs ischemic preconditioning group(N-WIPC, n =12).Changes in left ventricular functions,myocardial infarct size,creatine kinase(CK) in serum and MDA,SOD of myocardium were investigated. RESULTS:Compared with I/R group, non-wounded legs ischemic preconditioning and classical ischemic preconditioning could: (1)Reduce the myocardial infarct size( P 0 01);(2)Improve the left ventricular function;(3)Decrease the content of MDA of myocardium and CK in serum( P 0 01).Besides, the activity of SOD of myocardium increased in non-wounded legs ischemic preconditioning group. CONCLUSION:Non-wounded legs ischemic preconditioning has protective effects on the I/R myocardium approximating to classical ischemic preconditioning.