Cloning and Sequence Analysis of HN Protein Gene of Newcastle Disease Virus Strain Siping

The NDV strain Siping was grown in chicken embryos. The virus was purified and the genomic RNA was extracted. Using a pair of special primers,whole sequence of HN gene of NDV strain Siping was amplified by RT_PCR and sequenced after being directly inserted in plasmid KS(_).The sequence analysis showed that the nucleotide sequence of this HN gene was 1742 bp long encoding a protein of 571 amino acids. In the amino acid sequence,there are 5 glycosylation sites and 12 cysteine residues.The homology of the nucleotide is 83.4%～89.2% and that of the deduced amino acid sequence is 87.6%～91.1%.