OPTIMIZATION OF FLOCCULATING CONDITIONS OF MICROBIAL FLOCCULANT USING RESPONSE SURFACE METHODOLOGY
YU Han-qing
Abstract
YU Han-qing
Abstract
A bacterium TF10 was found to produce microbial flocculant P-TF10,and was identified as Bacillus megatherium.The effect of pH,flocculating dosage and various cations on flocculating activity was investigated against kaolin suspensions.The results showed that optimum pH and flocculating dosage exist for the flocculating efficiency.Mg2+ and Na+ had no effect on flocculation,while Ca2+ and Al3+ enhanced the flocculating activity.The response surface methodology was employed to optimize the flocculation process.The best flocculating efficiency(95.5±1.0)% was obtained with pH 4.7,CaCl2 dosage of 7.0mmol·l-1and P-TF10 dosage of 34.6 mg·l-1.
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A bacterium TF10 was found to produce microbial flocculant P-TF10,and was identified as Bacillus megatherium.The effect of pH,flocculating dosage and various cations on flocculating activity was investigated against kaolin suspensions.The results showed that optimum pH and flocculating dosage exist for the flocculating efficiency.Mg2+ and Na+ had no effect on flocculation,while Ca2+ and Al3+ enhanced the flocculating activity.The response surface methodology was employed to optimize the flocculation process.The best flocculating efficiency(95.5±1.0)% was obtained with pH 4.7,CaCl2 dosage of 7.0mmol·l-1and P-TF10 dosage of 34.6 mg·l-1.
Key concepts: Flocculation, Chemistry, Response surface methodology, Chromatography, Pulp and paper industry, Nuclear chemistry, Organic chemistry, Engineering