Study on the method of reversed-phase high performance liquid chromatography used for monitoring synthesis of sucrose-6-acetate

A method was established of reversed-phase high performance liquid chromatography for the determination of sucrose-6-acetate.In the method,Supelcosil~(TM)LC-18(250mm×4.6mm i.d.5μm)was used as chromatographic column and V(acetonitrile)∶V(methanol)∶V(water)=10∶10∶80 as mobile phase.The linear ranges was 0.5mg/L～900mg/L.The lower limit of detection of surose-6-acetate was 0.2mg/L.The recovery of the method was 98.53% and the relative standard deviations was 0.95%.By the method,the reaction raw materials,the reaction mediates and the reaction products could be separated effcctively,and the reaction progress could also be indicated directly.