P16 Expression and Its Correlation With Human Papillomavirus Infection in Cervical Lesions

Objective To evaluate the expression and correlation among p16 protein,infection of human papillomavirus (HPV) in invasive cervix carcinoma (ICC) and cervical intraepithelial neoplasia (CIN).Methods The immunohistochemical staining SP method was used to detect the expression of p16 protein and HybriMax was used to examine types of human papillomavirus in 40 cases of invasive carcinoma of cervix(ICC group),20 cases of CIN Ⅰ (CIN Ⅰ group),30 cases of CINⅡ~Ⅲ (CINⅡ~Ⅲ group),and 20 cases of normal cervix tissues(control group).Results ①There was no positive expression of p16 protein in normal cervix tissues (control group),but the over expression rates of CIN Ⅰ group,CIN Ⅱ～Ⅲ group,and ICC group were 35.0%,83.3%,and 100.0%,respectively.There was a significant trend of increasing expression from normal cervix or CINⅠ to invasive carcinoma of cervix (P0.05).② The infection of high-risk human papillomavirus (HR-HPV) had been detected as negative in normal cervical samples,but 55.0% in CIN Ⅰ group,80.0% in CIN Ⅱ~Ⅲ group,92.5% in ICC group.HPV-16/-18 were predominant types(50%) of high-risk human papillomavirus infection in cervical intraepithelial neoplasia and invasive carcinoma of cervix tissue samples.③There were significant positive correlations between the expression of p16 protein and high-risk human papillomavirus in cervical intraepithelial neoplasia and invasive carcinoma of cervix tissue samples(P0.05).Conclusion Cervical intraepithelial neoplasia expresses p16 protein which has malignant proneness.The investigation of p16 protein may used as a supplementary test for screening and routine diagnosis of cervical lesions.The over expressions of p16 protein may related with high-risk human papillomavirus infection.