Expression of MAGE-A4 in the Human Colorectal Neoplasm andCorrelation with Tumor Lymph Nodes Metastasis

Objective To study the expression of MAGE-A4 protein in the human colorectal neoplasm and to investigate the correlation with the invasion and metastasis of colorectal adenocarcinoma.Methods MAGE-A4 was detected at its protein level by SP-immunohistochemistry in 38 normal colorectal mucoses,31 colorectal adenoma and 83 colorectal adenocarcinoma.Results The expression of MAGE-A4 protein distributed in cytoplasm as well as nuclei.The positive rate of expression of MAGE-A4 protein was respectively 5.26%,38.71% and 38.55% in normal colorectal mucoses,colorectal adenoma and colorectal adenocarcinoma.There was significantly difference between the expression of MAGE-A4 protein in normal colorectal mucoses with colorectal adenoma or colorectal adenocarcinoma(P0.01).But the expression of MAGE-A4 protein had no significantly difference between colorectal adenoma and colorectal adenocarcinoma(P0.05).The expression of MAGE-A4 had yet no significantly difference(P0.05) in the different histological types of colorectal adenoma.The expressions of COX2 were not correlated with some clinicopathological indices (P0.05),such as gender,age,depth of invasion and liver metastasis of colorectal adenocarcinoma,etc.The expression of MAGE-A4 protein between colorectal adenocarcinoma with no regional lymph node metastasis and colorectal adenocarcinoma with regional lymph node metastasis was significantly different(P0.01,γs=0.312).Conclusion MAGE-A4 protein may play an important role in colorectal carcinogenesis and MAGE-A4 protein between colorectal adenocarcinoma with no regional lymph node metastasis and colorectal adenocarcinoma with regional lymph node metastasis was positive correlation.