Expression of COX-2 in Human Colorectal Tumors and Its Relation to Lymph Node Metastasis

Objective To investigate the effect of COX-2 protein in the carcinogenesis,progression,and metastasis of human colorectal adenocarcinomas.Methods COX-2 protein was detected by SPimmunohistochemistry in formalin-fixed and paraffin-embeded tissues of 30 colorectal adenoma tissues,35 colorectal adenocarcinoma tissues with no regional lymph nodes metastasis,and 35 primary colorectal adenocarcinoma tissues with regional lymph nodes metastasis.Results The expressions of COX-2 protein distributed in cytoplasms and cell membranes,it was gradually increased from colorectal adenoma tissues to colorectal adenocarcinoma tissues(P=0.000).From the well differentiated colorectal adenocarcinoma tissues to the poor differentiated tissues the expressions of COX-2 protein were significantly increased(P=0.009).And the expressions of COX-2 protein between colorectal adenocarcinoma tissues with no regional lymph nodes metastasis and primary colorectal adenocarcinoma tissues with regional lymph nodes metastasis had significantly difference(P=0.035).The expressions of COX-2 were not correlated with some clinicopathological indices(P0.05),such as gender,age,site of colorectal adenocarcinoma,et al.Conclusions COX-2 can be regard as markers for predicating the metastasis and invasion of the colorectal adenocarcinoma.So it was a well prognostic parameter for colorectal cancer.