Optimization of the extraction technique for proanthocyanidin from grape seeds with response surface methodology

The optimized extraction conditions for proanthocyanidin from grape seeds were determined by using the single factor experiments and response surface analysis.Solid-liquid ratio,extraction temperature and concentration of ethanol were taken as independent variables and yield of proanthocyanidin was taken as response value based on single factor tests.The results showed that the optimum extraction conditions were as follows:liquid ratio 1∶11.15,temperature 55.6 ℃ and ethanol concentration 72.8%.Under the optimal process,the extraction rate of proanthocyanidin reached 4.452%.