Optimization of Ultrasound-assisted Extraction of Proanthocyanidin from Rapeseed Hull Using Response Surface Methodology

Ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using ethanol as the extraction solvent was studied.Based on the single factor tests of ethanol volume fraction,extraction time,liquid-solid ratio and ultrasound power,the optimum process was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis.The results indicate that ethanol volume fraction of 62.9%,extraction time of 20.8 min and liquid-solid ratio of 25 mL/g were found to be the optimum conditions under study.Under these optimized conditions,the experimental value of proanthocyanidin extraction yield was 5.055 mg/g,in exact accordance with the predicted value 5.02 mg/g.Therefore,the ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology is feasible and reliable.