Detection and Drug-Resistance Analysis of Extended-Spectrum β-Lactmases-Producing Klebsiella Pneumoniae

Objective:To investigate the productive rates and antibiotic resistance characteristics of extended-spectrum β-lactamases(ESBLs)-producing Klebsiella Pneumoniae isolated from clinical strains.Methods: ESBLs-producing strains were determined by disk diffusion screening test and confirmatory test of NCCLS;Antibiotic susceptibility test were performed by Kerby-Bauer disk diffusion method.Results: 28 ESBLs-producing strains were determinded in confirmatory test,the rate of ESBLs production was 35.4%;The resistant rates of ESBLs-producing strains to 11 kinds of antibiotics were higher than those of non-ESBLs-producing strains,there were differences between the resistant rates of ESBLs-producing strains and those of non-ESBLs-producing strains(P0.05). There were not differences between the resistant rates of ESBLs-producing strains to 4 kinds of antibiotics and those of non-ESBLs-producing strains(P0.05).The resistant rates of ESBLs-producing strains and non-ESBLs-producing strains to imipenem were 100%.Conclusions: The situation of ESBLs-producing K-Pneumoniae is severe in this area;In most of antibiotics above,the resistant rates of ESBLs-producing K·Pneumoniae are significantly higher than those of non-producing-ESBLs.Imipenem,Cephamycins and β·lactamases inhibitor combinations can be used to deal with ESBLs-producing K·Pneumoniae infetion.