Apoptotic effect of oridonin on EJ cells and its mechanisms

Objective To study the antineoplastic effect of oridonin on bladder cancer cell lines,EJ cells in vitro.Methods The inhibitory rate of the cells was measured by MTT assay.Morphology of cell apoptosis was observed by Hoechst 33258 fluorescence staining and electron microscopy.Bcl-2 and bax expressions were detected by Western Blot.Telemerase activity was detected by telemerase repeat amplification proctocol(TRAP)-polymerase chain reaction(PCR)-silver stainning.Results Oridonin inhibited the growth of EJ cells and caused apoptosis significantly.The suppression was in a time-and dose-dependent manner.Oridonin down-regulated the bcl-2 expression and up-regulated the bax expression of EJ cells,decreased the activity of telemerase with time.Conclusion Oridonin has apparent inhibition and apoptosis-inducing effect on EJ cells,which may induce apoptosis via down-regulating Bcl-2/Bax and decreasing activity of telemerase in EJ cells.