Apoptotic effect of oridonin on T24 cells and its mechanisms

【Objective】To study the antineoplastic effect of oridonin on bladder cancer cell lines,T24 cells in vitro.【Methods】The inhibitory rate of the cells was measured by MTT assay.Morphology of cell apoptosis was observed by Hoechst 33258 fluorescence staining and light microscopy.Cell apoptotic rate was detected by flow cytom-etry(FCM) using Annexin V-FITC and propidium iodide(PI) detection kit.Caspase 3 was assayed by colorimetric assay kit.Bcl-2 and bax expressions were detected by Western Blotting.【Results】Oridonin inhibited the growth of T24 cells and caused apoptosis significantly.The suppression was both in a time-and dose-dependent manner.Oridonin increased the activity of Caspase 3,down-regulated the bcl-2 expression and up-regulated the bax expression of T24 cells with time.【Conclusions】Oridonin has apparent inhibition and apoptosis-inducing effect on T24 cells.Oridonin may induce apoptosis via down-regulating Bcl-2/Bax and increasing activity of Caspase 3 in T24 cells