Protective Effect of PCF on H_2O_2-induced HaCaT Cell Injury

[Objective]To establish in vitro model of H2O2-induced oxidative stress damage of HaCaT cells,and investigate the polypeptide from Chlamys farreri's protective effect and its possible mechanism on H2O2-induced damage to HaCaT cells. [Method]Cell Counting Kit-8( CCK-8) was used to test the cell survival rate of different concentrations( 50,100,200,300,500 μmol/L) H2O2for 12 h on HaCaT cells;different concentrations( 1. 42,2. 84,5. 68 mmol /L) PCF treated cell for 12 h,and then the appropriate H2O2concentration( 300 μmol /L)were adopted to treat,CCK-8 was used to detect the cell viability. Enzyme biochemical method was applied to detect the activity of LDH in culture supernatants,GSH-Px,T-AOC and CAT levels in cell lysis solution. [Result] Compared with control group,at 50- 500 μmol /L,H2O2caused concentration-dependent oxidative damage in the HaCaT cell,at 300 μmol /L H2O2decreased the cell survival rate to 56%( P 0. 01); Compared with model group,all different concentration PCF protected HaCaT cells from oxidative damage induced by H2O2,significantly increased the cell survival rate,decreased the release of LDH and increase GSH-Px,T-AOC and CAT level,enhanced antioxidation effect of HaCaT cell. [Conclusion]PCF has the significant protective effects on HaCaT cells injured by H2O2,which may be associated with the increased of antioxidase activity.