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Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A.

Ruyong Yao, Chunbo Wang

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Abstract

AIM: To study the protective effect of polypeptide isolated from Chlamys farreri (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro. METHODS: Cell proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM). RESULTS: PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA and the rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and control group were significant (P<0.05, P<0.01). CONCLUSION: PCF protected Hela cells against damage by UVA via its anti-oxidative mechanisms.

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What this paper is about

AIM: To study the protective effect of polypeptide isolated from Chlamys farreri (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro. METHODS: Cell proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM). RESULTS: PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA and the rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and control group were significant (P<0.05, P<0.01). CONCLUSION: PCF protected Hela cells against damage by UVA via its anti-oxidative mechanisms.

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Available abstract

AIM: To study the protective effect of polypeptide isolated from Chlamys farreri (PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro. METHODS: Cell proliferation was determined by MTT method; intra-cellular free calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry (FCM). RESULTS: PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase (GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell proliferation. The concentration of [Ca2+]i was increased while the amounts of MDA and the rates of apoptosis and death of the cells were decreased. The differences between the PCF groups and control group were significant (P<0.05, P<0.01). CONCLUSION: PCF protected Hela cells against damage by UVA via its anti-oxidative mechanisms.

Key concepts: HeLa, Apoptosis, Superoxide dismutase, Catalase, Flow cytometry, Oxidative stress, Molecular biology, MTT assay

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