Influences research of sulforaphen on HaCaT cells

Objective To discuss the influence of sulforaphen (SF) extracting from broccoli sprouts on HaCaT cells. Toprimary understand the safety of SF on HaCaT cells. Methods Subconfluent HaCaT cells were incubated for 24 hours with different doses of SF. MTT assay was used to test the viability of HaCat cell .A specific ELISA to detect concentration of tumor necrosis factor-α,and a flow cytometry was used to measured apoptotic rate. Results The concentration of SF above 200nmol/L reduced the viability of HaCat cell (P0.05).The SF of 50,100,150nmol/L had noany influence on the viability of HaCat cell,the level of the concentration of tumor necrosis factor-α,and the apoptotic rateof HaCaT cells (P0.05).But the apoptotic rate of the group of 200nmol/L was increased. Conclusion The viability of HaCat cell was not influenced by the SF of 50,100,150nmol/L. HaCaT cells were inhibited by the SF which above 200nmol/L concentration.