Mutation and selection of β-glucosidase producer
Qian He
Abstract
Qian He
Abstract
An Aspergillus ficuum strain producing β-glucosidase was obtained after the treatment of UV,NTG and Co~ 60 ,and the enzyme activity was increased. The culture medium and fermentation conditions were also optimized,and the productivity of the mutant N-9 in liquid state fermentation was increased by 2.1 fold, with the activity of 8.5U/mL.
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An Aspergillus ficuum strain producing β-glucosidase was obtained after the treatment of UV,NTG and Co~ 60 ,and the enzyme activity was increased. The culture medium and fermentation conditions were also optimized,and the productivity of the mutant N-9 in liquid state fermentation was increased by 2.1 fold, with the activity of 8.5U/mL.
Key concepts: Fermentation, Mutant, Food science, Chemistry, Strain (injury), Enzyme, Solid-state fermentation, Enzyme assay