2005Anhui nongye kexueRequires access

Selection of High-yield β-glucosidase Strain and the Optimized of Solid State Fermentation

Yang Jian-hai

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Abstract

The Aspergillus niger mutant strain was been selected by alternative treating Aspergillus niger with UV, EDS and 60Co. The enzymeactivityofβ-glucosidase fromfermentation ofthe mutant was heightened compared with the original. Further more the fermentation conditions forthe mutant were optimized through single factor and orthogonal experiments. Under the optimal condition, the enzyme activity of β-glucosidaseattained 169.2 U/ml.

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What this paper is about

The Aspergillus niger mutant strain was been selected by alternative treating Aspergillus niger with UV, EDS and 60Co. The enzymeactivityofβ-glucosidase fromfermentation ofthe mutant was heightened compared with the original. Further more the fermentation conditions forthe mutant were optimized through single factor and orthogonal experiments. Under the optimal condition, the enzyme activity of β-glucosidaseattained 169.2 U/ml.

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Available abstract

The Aspergillus niger mutant strain was been selected by alternative treating Aspergillus niger with UV, EDS and 60Co. The enzymeactivityofβ-glucosidase fromfermentation ofthe mutant was heightened compared with the original. Further more the fermentation conditions forthe mutant were optimized through single factor and orthogonal experiments. Under the optimal condition, the enzyme activity of β-glucosidaseattained 169.2 U/ml.

Key concepts: Aspergillus niger, Mutant, Fermentation, Solid-state fermentation, Yield (engineering), Strain (injury), Enzyme, Selection (genetic algorithm)

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