Association of rs388915 polymorphism of AT1R gene withessential hypertension

Objective:To investigate the possible genetic associations between the rs388915 polvmorphisms of angiotensin Ⅱ type 1 receptor(AT1R) gene and essential hypertension in Northen Han Chinese.Give theory to support the diagnostic,the cure and the research of hypertension.Methods:Essential hypertensive patients(n=640) and healthy normotensive subjects(n=442) were screened for the polymorphism of rs388915 by real-time quantitative polymerase chain reaction after DNA extraction to identify representative variations in the AT1R gene.Results:Hypertension patients and normotensive subjects were significantly different with respect to the genotypes GG,AG and AA: 23,211 and 393 vs.8,125 and 305(P=0.03).The allele frequency was also significantly different between the EH and NT groups(G,A=20.5%,79.5% vs.16.1%,83.9%).Similarly,there were significantly differences in dominant model and additive model between the two groups,but not in ressecive model.Further analyzed by gender,the number of GG,AG,AA and the frequencies of G allele and A allele between EH and NT females were respectively 7,87,132 vs.3,51,127 and 28.7%,71.3% vs.15.7%,84.3%,which differed significantly(P=0.045,P=0.000).As in male groups,there were no significant differences at all.The Logistic regression analysis,rs388915 allele is closely related with the pathogenesis of EH(P=0.024,OR=1.341,95% Cl=1.039-1.1731).Conclusion:rs388915 of AT1R gene is maybe associated with essential hypertension in the Han Chinese population,and especially is closely related with female hypertension other than in males.