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2006•The Chinese Journal of Modern Applied PharmacyRequires access

Comparison of hyperoside in folium apocyni veneti of different species and areas by HPLC

Qing‐Shan Li

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Abstract

OBJECTIVE To establish a method for determination of hyperoside in Luobuma by RP-HPLC and compare the difference between samples from different resources.METHODS The assay was performed on Shim-Pack VP-ODS column,acetonitrile-tetrahydrofuran-0.1% acetic acid(15.5∶5.5∶79)was used as mobile phase,the rate of flow was 1.0 mL/min,detection wavelength was 365nm,temperature of column was 30℃. RESULTS There was a good liner relationship within the concentration range of 0.01~(0.2) mg·mL~(-1)(r=0.999 9).The average recovery of hyperoside was 101.5% and RSD was 2.1%(n=6).The content of hyperoside was 0.60~0.91% in samples from Apocynum linn,and 0.39~0.55% in those from Poacynum baill.CONCLUSION The content of hyperoside in two species of Luobuma was found to be different.The method developed is reliable and accurate and can be used for quality control of folium apocyni veneti.

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OBJECTIVE To establish a method for determination of hyperoside in Luobuma by RP-HPLC and compare the difference between samples from different resources.METHODS The assay was performed on Shim-Pack VP-ODS column,acetonitrile-tetrahydrofuran-0.1% acetic acid(15.5∶5.5∶79)was used as mobile phase,the rate of flow was 1.0 mL/min,detection wavelength was 365nm,temperature of column was 30℃. RESULTS There was a good liner relationship within the concentration range of 0.01~(0.2) mg·mL~(-1)(r=0.999 9).The average recovery of hyperoside was 101.5% and RSD was 2.1%(n=6).The content of hyperoside was 0.60~0.91% in samples from Apocynum linn,and 0.39~0.55% in those from Poacynum baill.CONCLUSION The content of hyperoside in two species of Luobuma was found to be different.The method developed is reliable and accurate and can be used for quality control of folium apocyni veneti.

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Available abstract

OBJECTIVE To establish a method for determination of hyperoside in Luobuma by RP-HPLC and compare the difference between samples from different resources.METHODS The assay was performed on Shim-Pack VP-ODS column,acetonitrile-tetrahydrofuran-0.1% acetic acid(15.5∶5.5∶79)was used as mobile phase,the rate of flow was 1.0 mL/min,detection wavelength was 365nm,temperature of column was 30℃. RESULTS There was a good liner relationship within the concentration range of 0.01~(0.2) mg·mL~(-1)(r=0.999 9).The average recovery of hyperoside was 101.5% and RSD was 2.1%(n=6).The content of hyperoside was 0.60~0.91% in samples from Apocynum linn,and 0.39~0.55% in those from Poacynum baill.CONCLUSION The content of hyperoside in two species of Luobuma was found to be different.The method developed is reliable and accurate and can be used for quality control of folium apocyni veneti.

Key concepts: Hyperoside, Folium of Descartes, Chromatography, Chemistry, High-performance liquid chromatography, Rutin, Biochemistry, Antioxidant

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