Comparison of anti-tumor drugs chemosensitivity assays

Objective To investigate the application of flow cytometry in the assessment of anti-tumor drugs chemosensitivity.[WT5”HZ]Methods Sub-G_1 and Annexin V assays were used to detect the apoptosis of Molt-4 cell line and fresh gastrointestinal cancer cells which were exposed to anti-tumor drugs,such as 5-fluorouracil and Paclitaxel.The outcomes were compared with the ones from MTT colormetric assay.Results[WT5”BZ] The outcome of Annexin V assay or Sub-G_1 assay was closely associated with MTT assay.But the time point of the maximum cytotoxicity of drugs detected by Annexin V assay was earlier than that by MTT assay or by Sub-G_1 assay.Conclusion Annexin V and Sub-G_1 assays could be used as the effective method to test the anti-tumro drugs chemosensitivity.Annexin V assay would be a sensitive,rapid,reliable approach for measuring anti-tumor drugs chemosensitivity.