Measurement and Analysis of TNF-α in Gingival Crevicular Fluid from Type 2 Diabetes Complicated with Periodontal Diseases Patients

Objective: To compare the TNF-α level in gingival crevicular fluid(GCF) from periodontal diseases in type 2 diabetes mellitus with which from systemically healthy person and the relationship between TNF-α concentration and metabolic condition.Methods: Thirty diabetics complicated with periodontal diseases patients and periodontal diseases and thirty systemically healthy persons were included in this study.Their TNF-α was measured by radioimmunoassay,and HbA1c levels of diabetic patients were measured by high-performance liquid chromatography.Results: The diabetics with periodontal diseases and patients with periodontal diseases had higher GCF and GCF-TNF-α as compared to the control group(P0.01).The diabetics with periodontal diseases had higher GCF-TNF-α as compared to the periodontal diseases group(P0.01).The levels of TNF-α of diabetics with periodontal diseases did not correlate with HbA1c levels.Conclusion: The TNF-α level was controlled by systemic factor.The high TNF-α contents in diabetics may be a consequence of a systemic abnormal response and a more severe periodontal disease may thus be resulted.