Effects of Electroacupuncture on nerve growth guidance factor Slit2 of Rats after Sciatic Nerve Injury

Objective To approach to the effects on nerve growth guidance factor Slit2 of rats after sciatic nerve injury. Methods 72 healthy SD rats were randomly divided into control group,model group the electroacupuncture(EA) group(n = 24).In the latter two groups,the right sciatic nerve was transected end-to-end suture immediately.The treatment of EA on Huantiao(GB 30) and Zusanli(ST 36) was applied once daily,and 7 days a course of treatment,continuous treatment of three courses.The changes in expression of Slit2 Slit2 mRNA in sciatic nerve corresponding section of spinal cord were detected by RT-PCR immunohistochemical methods after the end of each course of treatment. Results EA group compared with model group,Slit2 its mRNA in the injured rat sciatic nerve spinal cord segments reached a peak after the first course of treatment,then decreased gradually,and are higher than the model group,there was a remarkably significant difference(P0.01),and the two groups is always higher than the control group,there are also remarkably significant differences(P0.01). Conclusion EA treatment can remarkably strengthen expression of Slit2 Slit2 mRNA in sciatic nerve corresponding section of spinal cord after sciatic nerve injury.This may be the one of the mechanisms for the treatment of peripheral nerve injury.