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Effect of Platelet-derived Growth Factor on Proliferation ofHypoxic Pulmonary Artery Smooth Muscle cell

Pu M

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Abstract

By means of flow cytometry and in situ hybridization ,we investigated the role of hypoxia in cultured neonatal bovine pu,lmonary artery smooth muscle cells (PASMC ) ,and the effect of PDGF on that role. Cell-cycle analysis revealed hypoxia ( 12h ) and PDGF added meantime had effect on the G/G S transition , both could increase the number of cells entering S phase from G/G The percents of S phase cells reached to 41%and 63%respectivly , and werel 57. 7% and 2.2. 3% compared with normoxiit (P0. 05). Using in situ hybridization , we detected that hypoxia (12h ) upregulated PDGF-A,B chain and receptor gene expres- sion (all P0. 001). Results therefore suggested that increase of PDGF-A ,B chain and receptor gene elxpression under hypoxia condition and effect of PDGF cooperated with hypox- ia on PASMCs proliferation may contribute to the pulmonary vascular remodeling.

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What this paper is about

By means of flow cytometry and in situ hybridization ,we investigated the role of hypoxia in cultured neonatal bovine pu,lmonary artery smooth muscle cells (PASMC ) ,and the effect of PDGF on that role. Cell-cycle analysis revealed hypoxia ( 12h ) and PDGF added meantime had effect on the G/G S transition , both could increase the number of cells entering S phase from G/G The percents of S phase cells reached to 41%and 63%respectivly , and werel 57. 7% and 2.2. 3% compared with normoxiit (P0. 05). Using in situ hybridization , we detected that hypoxia (12h ) upregulated PDGF-A,B chain and receptor gene expres- sion (all P0. 001). Results therefore suggested that increase of PDGF-A ,B chain and receptor gene elxpression under hypoxia condition and effect of PDGF cooperated with hypox- ia on PASMCs proliferation may contribute to the pulmonary vascular remodeling.

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Available abstract

By means of flow cytometry and in situ hybridization ,we investigated the role of hypoxia in cultured neonatal bovine pu,lmonary artery smooth muscle cells (PASMC ) ,and the effect of PDGF on that role. Cell-cycle analysis revealed hypoxia ( 12h ) and PDGF added meantime had effect on the G/G S transition , both could increase the number of cells entering S phase from G/G The percents of S phase cells reached to 41%and 63%respectivly , and werel 57. 7% and 2.2. 3% compared with normoxiit (P0. 05). Using in situ hybridization , we detected that hypoxia (12h ) upregulated PDGF-A,B chain and receptor gene expres- sion (all P0. 001). Results therefore suggested that increase of PDGF-A ,B chain and receptor gene elxpression under hypoxia condition and effect of PDGF cooperated with hypox- ia on PASMCs proliferation may contribute to the pulmonary vascular remodeling.

Key concepts: Platelet-derived growth factor receptor, Hypoxia (environmental), Flow cytometry, In situ hybridization, Platelet-derived growth factor, Growth factor, Cell growth, Downregulation and upregulation

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Effect of Platelet-derived Growth Factor on Proliferation ofHypoxic Pulmonary Artery Smooth Muscle cell — Research Paper | ScholarLens