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[Platelet-derived growth factor (PDGF) mRNA expression of porcine pulmonary artery smooth muscle cells induced by hypoxic endothelial cells conditioned medium].

Li F, Y Zhang, Dongsheng Che

Abstract

OBJECTIVE: To study the effect of hypoxic endothelial cell conditioned medium on the autocrine of PDGF in porcine pulmonary artery smooth muscle cells (PASMC). METHODS: Experimental groups included serum-free medium group (control group), normoxic endothelial cell conditioned medium (NECCM) group and hypoxic endothelial cell conditioned medium (HECCM) group. The expression of PDGF-A and -B chain mRNA was determined by in situ hybridization and image analysis. RESULTS: A weak expression for PDGF-A and PDGF-B mRNA was observed in PASMC cultured in serum free medium (control group); HECCM markedly enhanced the expression of PDGF-A and PDGF-B mRNA in PASMC, both being about 1.8-fold and 1.73-fold of the control group respectively, and 1.4-fold and 1.5-fold of the NECCM group respectively (P < 0.01). CONCLUSION: PDGF may be an autocrine growth factor of PASMC induced by HECCM and may play an important role in vascular structural remodeling of hypoxic pulmonary hypertension.

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Key concepts: Autocrine signalling, Platelet-derived growth factor receptor, Platelet-derived growth factor, Growth factor, In situ hybridization, Biology, Messenger RNA, Endocrinology