Determination of Notoginsenoside R_1,Ninsenoside Rg_1 and Ginsenoside Rb_1 in Naodesheng Soft Capsules by HPLC-ELSD

ObjectiveTo develop an HPLC-ELSD method for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rbl in Naodesheng soft capsules. MethodsAlltima C18 chromatography column was used with acetonitrile-water(0→20min,acetonitrile20%→40%) as mobile phase.The flow rate of mobile phase was 1.0 ml·min-1,the tube temperature of the detector was 40℃,and the press of pure air was 3.5 kPa. ResultsThe linear ranges were 0.150～3.00 μg(r=0.999 1)for notoginsenoside R1,0.753～15.06 μg(r=0.999 6)for ginsenoside Rg1 and 0.755～15.10 μg(r=0.999 5)for ginsenoside Rb1.The average recoveries were 98.41% with the corresponding RSD 1.4% for notoginsenoside R1,98.91% with corresponding RSD 1.2% for ginsenoside Rg1 and 99.34% with corresponding RSD 1.5% for ginsenoside Rbl,respectively. ConclusionThe method is simple,sensitive and reproducible and can be used for quality control of Naodesheng soft capsules.