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HPLC-ELSD determination of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Naodesheng tablets

Liu Yan-wen

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Abstract

Objective:To develop the quantitative method of notoginsenoside R1,ginsenoside Rgl,and Rbl in Naodesheng tablets by HPLC-ELSD.Method:The column was packed with 5 μm Hypersil ODS-C18 stationary phase.The mobile phase consisted of acetonitrile-water,eluted in gradient mode(0-20 min,acetonitrile 20%→40%;20-26 min,acetonitrile 40%→20%) with flow rate of 1.0 mL·min-1.Column temperature was set at 25 ℃.The temperature of driftube was 40 ℃ and the nebulizer nitrogen pressure was 3.5 kPa.Results:The linear ranges of notoginsenoside R1,ginsenoside Rgl and Rbl were 0.150-3.00 μg(r=0.9991),0.753-15.1 μg(r=0.9996) and 0.755-15.1 μg(r=0.9995),respectively;the average recoveries(n=6) were 98.3%,96.7%,98.6%,with the RSD of 1.0%,1.5%,0.9% respectively.Conclusion:The method is rapid,simple and accurate.It can be used for quality evaluation of Naodesheng tablets.

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What this paper is about

Objective:To develop the quantitative method of notoginsenoside R1,ginsenoside Rgl,and Rbl in Naodesheng tablets by HPLC-ELSD.Method:The column was packed with 5 μm Hypersil ODS-C18 stationary phase.The mobile phase consisted of acetonitrile-water,eluted in gradient mode(0-20 min,acetonitrile 20%→40%;20-26 min,acetonitrile 40%→20%) with flow rate of 1.0 mL·min-1.Column temperature was set at 25 ℃.The temperature of driftube was 40 ℃ and the nebulizer nitrogen pressure was 3.5 kPa.Results:The linear ranges of notoginsenoside R1,ginsenoside Rgl and Rbl were 0.150-3.00 μg(r=0.9991),0.753-15.1 μg(r=0.9996) and 0.755-15.1 μg(r=0.9995),respectively;the average recoveries(n=6) were 98.3%,96.7%,98.6%,with the RSD of 1.0%,1.5%,0.9% respectively.Conclusion:The method is rapid,simple and accurate.It can be used for quality evaluation of Naodesheng tablets.

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Available abstract

Objective:To develop the quantitative method of notoginsenoside R1,ginsenoside Rgl,and Rbl in Naodesheng tablets by HPLC-ELSD.Method:The column was packed with 5 μm Hypersil ODS-C18 stationary phase.The mobile phase consisted of acetonitrile-water,eluted in gradient mode(0-20 min,acetonitrile 20%→40%;20-26 min,acetonitrile 40%→20%) with flow rate of 1.0 mL·min-1.Column temperature was set at 25 ℃.The temperature of driftube was 40 ℃ and the nebulizer nitrogen pressure was 3.5 kPa.Results:The linear ranges of notoginsenoside R1,ginsenoside Rgl and Rbl were 0.150-3.00 μg(r=0.9991),0.753-15.1 μg(r=0.9996) and 0.755-15.1 μg(r=0.9995),respectively;the average recoveries(n=6) were 98.3%,96.7%,98.6%,with the RSD of 1.0%,1.5%,0.9% respectively.Conclusion:The method is rapid,simple and accurate.It can be used for quality evaluation of Naodesheng tablets.

Key concepts: Chemistry, Chromatography, High-performance liquid chromatography, Ginsenoside, Ginsenoside Rg1, Acetonitrile, Gradient elution, Chromatography detector

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HPLC-ELSD determination of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Naodesheng tablets — Research Paper | ScholarLens