Applicable value of combined detection of three methods in diagnosis of senile syphilis

Objective To explore the applicable value of combined detection of three methods [enzyme linked immunosorbent assay(ELISA),syphilis toluidine red untreated serum test(TRUST)and treponemapallidum particle agglutination assay(TPPA)] in diagnosis of senile syphilis.Methods The clinical specimens underwent routine treponema pallidum antibody screening with ELISA method.All ELISA positive samples were further confirmed by TRUST and TPPA.Results Among 78 treponema pallidum antibody screening positive specimens,53 samples were confirmed positive by TPPA,and the positive coincidence rate of ELISA and TPPA was 67.95%;in which 51 cases were both TRUST and TPPA.A total of 27 cases were TRUST negative and TPPA positive.In 27 cases with TRUST negative results,25 cases were TPPA positive and 2 cases were TPPA negative.Conclusion High rate of ELISA false positive result existed in senile syphilis screening test.ELISA screening positive specimens should be firstly detected with TRUST.All TRUST negative samples by ELISA should be further tested by TPPA so as to reduce the misdiagnosis and medical dispute in diagnosis of senile syphilis.