Microsatellite marker analyses on genetic diversity and phylogenetics in Gansu local breeds of sheeps

Using 15 microsatellite DNA markers,6 Gansu local breeds of sheep were selected to study their genetic diversity and phylogenetics.Results demonstrated that 15 microsatellite loci of 240 individuals had 179 alleles discovered.The number of alleles in Tibetan sheep was 136 as the most,and Lanzhou fat tailed-sheep was only 94 as the least.The Populations PIC and heterozygosity analysis showed that Tibetan sheep and Gansu Alpine Merino had rich genetic diversity,but the genetic diversity of Lanzhou fat tailed-sheep and Minxian Black Fur sheep was lower than others in concerned 6 sheep breeds.The genetic distance and phylogenetic tree result shows that the Lanzhou fat tailed-sheep,Tan sheep and Minxian Black Fur sheep relationship are nearer.They have the same ancestor.In addition,the relationship between Gansu Alpine Merino and the other 5 sheep breeds were very far than the others.