Analysis of microsatellite polymorphism in five sheep populations

In order to evaluate the genetic diversity and relationship of Sannan Gansu Alpine Merino,Tianzhu Gansu Alpine Merino,Tan sheep,Small Tailed Han sheep and Tibetan sheep,five microsatellite DNA marks were used in five sheep populations(125 individuals).The results showed that the number of alleles detected in Tianzhu Gansu Alpine Merino are most,was 57,and the least in Tan sheep,was only 43.The populations PIC and heterozygosity analysis showed that Tianzhu Gansu Alpine Merino and Sunan Gansu Alpine Merino had the richest genetic diversity,but Tan sheep genetic diversity was lower than others.The results of genetic distance and phylogenetic tree analysis showed that the relationship of Sannan Gansu Alpine Merino,Tianzhu Gansu Alpine Merino and Tibetan sheep was nearer.Moreover,the relationship of Small Tailed Han sheep and Tan sheep was nearer.