Microsatellite DNA analysis on genetic diversity in five sheep breeds

The genetic diversity in 5 sheep breeds(Tibet sheep,Lanzhou large-tailed sheep,Mongolia sheep,Small-tailed Han sheep and Poll Dorset sheep) was analyzed by using 8 microsatellite DNA markers.The results indicated that,105 alleles for 8 microsatellite loci in 5 sheep breeds were discovered,the average allele number on a locus was 13.There were the most number of alleles in OarFCB128,and the least in BM8125.The data of polymorphism information contents(PIC),effective number of alleles(Ne) and heterozygosity(He) indicated that,the genetic variation of Small-tailed Han sheep was the highest,Mongolia sheep was the second,Lanzhou large-tailed sheep was the third,Tibet sheep was the fourth,Poll Dorset sheep was the lowest.According to the Nei genetic distances,the clustering figures were established through the NJ tree and UPGMA tree,the results showed that Lanzhou large-tailed sheep and Mongolia sheep were clustered together,whose genetic distances was the nearest,the genetic distances between Lanzhou large-tailed sheep and Poll Dorset sheep was the farthest.