Study on DNA damages of spodoptera litura SL-1 cells induced by formaldehyde

To study the effect of formaldehyde inducing DNA-protein crosslinks(DPC) and DNA single strand break(DSSB) in insect cells,this study took Spodoptera litura SL-1 cells as experimental material and made use of KCl-SDS assay and comet assay to determine the amount of DNA-protein crosslinks and effect of DNA damages induced by liquid formaldehyde.From the KCl-SDS assay results formaldehyde can not cause DPC at low levels(25 μmol/L,125 μmol/L),but at higher levels(625 μmol/L) can cause DPC obviously(P0.01).According to comet assay,it can cause DNA strand breakage(DSSB) at low levels(5 μmol/L,25 μmol/L,P0.01) and cause crosslinks at higher levels(625 μmol/L,P0.01).From the above results this paper can conclude that formaldehyde can cause DPC at higher,at lower levels the mostly action is DSSB.