Study on DNA damages of Ovomermis sinensis induced by formaldehyde

To explore the effect of DNA-protein crosslinks (DPC) and DNA strand breakage(DSB) induced by formaldehyde in nematode,this study took Ovomermis sinensis as experimental material and made use of KCl-SDS assay and comet assay to determine the amount of DNA-protein crosslinks and effect of DNA strand breakage , with exposure to liquid formaldehyde in vivo. From the results of KCl-SDS assay formaldehyde at low levels (5, 25, 125 μmol·L -1 ) can not cause DPC significantly (p0.05), but at higher levels (625μmol·L -1 ,p0.05) causes DPC obviously. According to the results of comet assay, it can cause DNA strand breakage (DSB) at low levels (5 and 25μmol·L -1 p0.01) and cause crosslinks at higher levels (125 and 625μmol·L -1 ,p0.01). From the above results we can conclude that formaldehyde can cause DPC at higher levels, at lower 25μmol·L -1 the general effect is DSB.