Screening of Antagonistic Bacterium for Control of Watermelon Fusarium Wilt
Su Xiaofei
Abstract
Su Xiaofei
Abstract
[Objective] The antagonistic bacteria which could resist watermelon fusarium wilt were screened from soil to control fusarium wilt biologically.[Method] The plate isolation was used to separate actinomycetes,fluorescent bacteria and bacillus sp,after purification,colonies were carried resistance selection through confront culture and the colonies with best antagonistic effect were identified and classified.[Result]29 strains with stable antagonistic effect on watermelon fusarium wilt were selected from 39 soil samples and 15 fluorescent bacteria,5 bacillus sp and 9 actinomycetes were among them.3 antagonistic bacteria (FM2,FM3 and FM4) with strongest antagonistic effect were conducted preliminary identification.[Conclusion] The cultural characteristics,morphological observation,physiological and biochemical determination of colonies demonstrated that FM2 was Bacillus Subtilis,while FM3 and FM4 were micrococcus.
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[Objective] The antagonistic bacteria which could resist watermelon fusarium wilt were screened from soil to control fusarium wilt biologically.[Method] The plate isolation was used to separate actinomycetes,fluorescent bacteria and bacillus sp,after purification,colonies were carried resistance selection through confront culture and the colonies with best antagonistic effect were identified and classified.[Result]29 strains with stable antagonistic effect on watermelon fusarium wilt were selected from 39 soil samples and 15 fluorescent bacteria,5 bacillus sp and 9 actinomycetes were among them.3 antagonistic bacteria (FM2,FM3 and FM4) with strongest antagonistic effect were conducted preliminary identification.[Conclusion] The cultural characteristics,morphological observation,physiological and biochemical determination of colonies demonstrated that FM2 was Bacillus Subtilis,while FM3 and FM4 were micrococcus.
Key concepts: Bacteria, Bacillus subtilis, Biology, Biological pest control, Fusarium wilt, Isolation (microbiology), Fusarium, Bacillus (shape)