Study on the growth promoting culture of Lactobacillus acidophilus
Chen Xiao-mei, Zhang Xun-jie, Luo Jian-ling, Chao Wu
Abstract
Chen Xiao-mei, Zhang Xun-jie, Luo Jian-ling, Chao Wu
Abstract
11% NFM medium was founded by screening several radical media to cultivate Lactobacillus acidophilus. Based on the 11% NFM medium, the optimal medium was obtained by orthogonality test consisted of tomato juice 5%,carrot juice 10%, liver infusion 0. 1%, beer 5%. Growth curve with optimized stimulated culture was determined by following the change of pH and titratable acidity. According to the growth curve. the terminal time was in 10 hours and Lactobacillus acidophilus got to 7. 49×10~9cfu/ml.
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11% NFM medium was founded by screening several radical media to cultivate Lactobacillus acidophilus. Based on the 11% NFM medium, the optimal medium was obtained by orthogonality test consisted of tomato juice 5%,carrot juice 10%, liver infusion 0. 1%, beer 5%. Growth curve with optimized stimulated culture was determined by following the change of pH and titratable acidity. According to the growth curve. the terminal time was in 10 hours and Lactobacillus acidophilus got to 7. 49×10~9cfu/ml.
Key concepts: Lactobacillus acidophilus, Titratable acid, Food science, Lactobacillus, Chemistry, Growth curve (statistics), Lactobacillaceae, Mathematics