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2009•Bulletin of Science and TechnologyRequires access

Study on High Cell Density Culture of Lactobacillus acidophilus

Guoqing He

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Abstract

This paper mainly studied the high cell density culture of Lactobacillus acidophilus,and discussed the effects of cultural condition(temperature,inoculation capacity and initial pH)and media composition.The optimal culturing conditions were:pH5.8,culture temperature37℃,4% inoculation capacity;culture composition of whey mist 2%,beef extract 1.5%,peptone 1.5%,corn,MgSO4 0.058%,MnSO4 0.025%,NaAC 0.022%,K2HPO40.2%.The amount of lactobacillus acidophilus was up to 1.17×1011CFU / mL for 22 hours based on the above conditions.

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What this paper is about

This paper mainly studied the high cell density culture of Lactobacillus acidophilus,and discussed the effects of cultural condition(temperature,inoculation capacity and initial pH)and media composition.The optimal culturing conditions were:pH5.8,culture temperature37℃,4% inoculation capacity;culture composition of whey mist 2%,beef extract 1.5%,peptone 1.5%,corn,MgSO4 0.058%,MnSO4 0.025%,NaAC 0.022%,K2HPO40.2%.The amount of lactobacillus acidophilus was up to 1.17×1011CFU / mL for 22 hours based on the above conditions.

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Available abstract

This paper mainly studied the high cell density culture of Lactobacillus acidophilus,and discussed the effects of cultural condition(temperature,inoculation capacity and initial pH)and media composition.The optimal culturing conditions were:pH5.8,culture temperature37℃,4% inoculation capacity;culture composition of whey mist 2%,beef extract 1.5%,peptone 1.5%,corn,MgSO4 0.058%,MnSO4 0.025%,NaAC 0.022%,K2HPO40.2%.The amount of lactobacillus acidophilus was up to 1.17×1011CFU / mL for 22 hours based on the above conditions.

Key concepts: Lactobacillus acidophilus, Inoculation, Food science, Probiotic, Composition (language), Chemistry, Lactobacillus, Microbiology

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