Enzymatic Hydrolysis and Butanol Fermentation of Pretreated Corn Stalk

[Objective] The aim was to find the optimum enzymolysis process conditions after corn stalk pretreatment.[Method] The corn stalk was pretreated by using alkali steeping method and ammonia steeping method to study the effect of the factors such as pretreatment method,temperature,enzyme concentration,pH value and substrate concentration on the corn stalk enzymatic hydrolysis,which got the optimum enzymolysis process conditions and used the optimum condition hydrolysate to make butanol fermentation.[Result] The alkali method could effective improve the hydrolysis efficiency of enzyme.The optimum enzymatic hydrolysis process condition after corn stalk pretreatment was that the pH value was 4.5-5.0,the temperature was 50 ℃,the substrate concn.was 3.33%,the enzyme dosage was 950 U/g stalk.The yield radio of solvents(butanol,acetone,ethanol) was 10.01.51.0 after making butanol fermentation by using stalk hydrolysate,which increased the radio of butanol,comparing with the traditional fermented(631).[Conclusion] This research provided the test basis for the development and utilization of new energy with lignocellulose as the material.