Fermentation Conditions of Endophytic Bacterium Strain 29-12 Biocontrolling Eggplant Verticillium dahliae

To work out the fermentation conditions of endophytic bacterium strain 29-12 of Bacillus subtilis antagonistic to Verticillium dahliae from eggplant,culture condition was optimized and orthogonal tests were designed.The optimal culture medium composition was showed as corn meal 15 g/L,poi starch 5 g/L,soybean meal 15 g/L,peptone 8 g/L,MgSO4 0.01%,and K2HPO4 0.05%.When the strain was cultured in above medium with the following conditions as initial pH 7.5,inoculation level 10%,a 120 r/min shaking revolution with 100 ml medium in 250 ml flask,culture temperature at 30 ℃ for 84 h,the diameter of inhibition zone reached 2.12 cm,the bacterial quantity in 1 ml was 1.58×109 and the spore production rate reached 100%.