Fermentation Condition Optimization of Endogenous Bacillus amyloliquefaciens X-278

Taking Bacillus amyloliquefaciens as test material,using single factor and orthogonal experiments,and through comparing biomass of fermentation broth and bacteriostatic activity against Verticillium dahliae Kleb,the fermentation conditions of endogenous Bacillus amyloliquefaciens X-278 were optimized.The results showed that the final optimization of the medium and conditions:1.5% glucose,2% peanut power,0.5%(NH4) 2 SO4,0.1% CaCO3,0.5% MgSO4,0.5% NaCl,pH 7,culture temperature 34℃,rotation speed 180r/min,seed liquid age was 24h,liquid volume was 150mL/500mL of triangular flask,inoculum size was 2%,incubation time was 72h.After optimizing,the number of viable bacteria was to 1.5×1010 CFU/mL in the fermentation broth,and the diameter of inhabiting circle against Verticillium dahliae was 33.4mm.